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Cultured cells were stimulated for 6 h with PMA (50 ng ml 1 ) (BioGems) and ionomycin (1 g ml 1 ) (BioGems) in the presence of brefeldin A (1 g ml 1 ) (GolgiPlug, BD Biosciences), stained for viability (Zombie UV Fixable Viability Dye (BioLegend)) and surface markers, fixed and permeabilized with 3.7% formaldehyde (Sigma) and 0.1% NP-40, respectively, and stained for intracellular cytokines

[PMC free article] [PubMed] [Google Scholar] 79.Amin G.R

Gross swelling is typically followed by rapid shrinking during the following days as GAGs (or their fragments) diffuse out of the disrupted and swollen tissue

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